Description:
UltraPure Agarose From Invitrogen, Cat Number: 15510-027, 500g.
UltraPure Agarose and InnoVita's GR Safe DNA Gel Stain combo offers these benefits:
- Saves money versus buying these same products separately
- Offers a safer and better alternative to ethidium bromide
Eliminates risks to yourself, the environment and your institution
UltraPure Agarose resolves DNA and RNA fragments from 100 bp to >30 kb.
UltraPure Agarose is a standard melting temperature, multi-purpose agarose that is ideal for routine separation analysis. Its high gel strength specification ensures that gels will not break during handling, or during Southern and northern transfers. UltraPure Agarose can also be used in some protein electrophoresis applications, such as Ouchterlony (antigen-antibody interaction assay) and radial immunodiffusion (RID) (antigen quantitation assay).
UltraPure Agarose is used for:
- Analytical separation of DNA, RNA, and PCR fragments >1,000 bp
- Recovery of DNA, RNA, and PCR fragments >1,000 bp
- Southern and northern blotting of fragments >1,000 bp
- Ouchterlony and radial immunodiffusion (RID)
GR Safe DNA Gel Stain provides sensitive DNA and RNA detection with substantially reduced mutagenicity, making it much safer to use than ethidium bromide. It is provided as a convenient ready-to-use solution that can be used either for post-staining or incorporated into gels. The detection sensitivity with GR Safe is comparable to that obtained with ethidium bromide. DNA bands stained with GR SafeDNA gel stain can be detected using a blue light transilluminator such as Invitrogen's Safe Imager, a standard UV transilluminator, or a laser-based scanner. Bound to nucleic acids, the green-fluorescent GR Safe stain has fluorescence excitation maxima at ~290 and ~490 nm, and an emission maximum at ~530 nm.
Performance and Quality Testing: Performance of UltraPure Agarose is evaluated by gel analysis using a control lot of nucleic acid molecular size standards. No DNase or RNase activity detected.
Storage:
Store UltraPure Agarose at 15oC to 30oC. GR Safe should be stored at room temperature in the original container.