Fast Electrophoresis Buffer (FEB Buffer) containing sodium acetate, sodium borate and boric acid, is optimized for rapid and high resolution separation of DNA on agarose or polyacrymide gels. FEB buffer allows for higher voltage runs, rapid running times (10-30 min), and high-resolution band separation.
1X FEB running buffer can be made by diluting 100 ml of 20X FEB with 900 ml ddH2O. A voltage of 120 V is recommended for small electrophoresis chambers while a mid to large apparatus may be run at 200-250 V. It is recommended that the user test their chamber to determine optimal voltage. Agarose gels should be prepared using 1X FEB Buffer.
FEB buffer is compatible with DNA staining using GRGreen or Ethidium Bromide, and downstream purification steps using Qiagen Gel Purification kit.
Gel image shows separation of 100bp ladder and 1kb ladder using FEB buffer. DNA was stained with GRGreen.
