Size: 1000 µl in water.
GR Safe is a nucleic acid stain for detecting nucleic acids, e.g., double-stranded DNA, in agarose gel. It can be used for replacing mutagenic ethidium bromide (EB).
Compared to EB which is a very strong mutagen, GR Safe caused fewer mutations than EB in the Ames test.
- Available at 10,000X in H2O for better safety --No more toxic and flammable organic solvent
- Stable. GR Safe can be stored at room temperature or 4 oC --No more freeze-and-thaw cycle!
- Compatible with UV or blue light transilluminator and common gel documentation systems.
- Will not affect downstream experiments: compatible with all gel purification kits tested, will not inhibit ligation reaction etc.
- Compatible with Sodium Borate Electrophoresis Buffer: Run gel 2-3 times faster at higher voltage, resolve shaper bands in minutes, and less heat generation.
- Cut out DNA bands for subclonning under safer blue light: No mutations caused by EB and UV light.
GR Safe vs EtBr
| GR Safe |
EtBr |
 |
|
Storage: Store at room temperature.
Protocol:
1. Prepare 20 to 40 ml of agarose gel solution (concentration from 0.7~2.0%) with TAE or Borate Buffer in a 250 ml flask and mix it thoroughly. Place the flask in the microware, heat on high until the solution is completely clear and no small floating particles are visible (about 2~3 minutes).
2. After the gel solution cool to about 55 oC, add 1 to 4 µl of GR Safe to the solution. Swirl the flask gently to mix the solution and avoid forming bubbles.
3. Pour the gel solution into a gel tray until the comb teeth are immersed about 1/4~1/2 into the gel solution.
4. After the agarose gel has solidified you can perform electrophoresis using either 1X TAE or 1X Borate Buffer.
5. Detect the bands using UV or blue light transilluminator.
FAQ
1, Should I wear gloves when using this dye?
You should exercise common safe laboratory practices when using this reagent.
2, What blue light transilluminator should I used with GR Safe dye?
DarkReader.
3, What filter should I use for blue light transilluminator?
Filter from Clare Chemical.
4, Can I use UV transilluminator?
UV transilluminator is good. GR Safe has lower background with UV transilluminator compared to blue light transilluminator.
Protocol: 
