GRGreen Loading Buffer contains GRGreen Nucleic Acid Stain, a highly sensitive fluorescent dye that enables instant band visualization during or immediately after running your agarose gel. Supplied in 6X loading buffer, it forms a tight complex with the sample nucleic acids and comigrates with nucleic acids during electrophoresis. After the run, simply place the gel on a standard UV or blue light transilluminator to view nucleic acids bands. No post staining or destaining is required.
GRGreen Loading Buffer can be used to prestain your DNA samples prior to sample loading. This prestaining treatment can dramatically increase band sharpness (eliminate smiley and smearing), resulting better resolution of DNA bands.
| No prestaining |
"Prestaining" with GRGreen |
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Storage and Handing
GRGreen Loading Buffer is supplied as a 6X gel loading buffer. For long-term storage the loading buffer should be stored at -20 ℃ protected from light. It is stable for 6 months at -20 ℃ and 1 month at 4 ℃.
GRGreen dye can be completely removed from nucleic acids by alcohol precipitation or Qiagen QIAquick Gel Extraction.
DNA Staining Protocol
Add 2 µl of the 6X loading buffer to 10 µl DNA sample and DNA marker/ladder. Load samples and run the gel using your standard protocol. View DNA bands using a UV or blue light transilluminator.
Protocol: GRGreen DNA Loading Buffer Protocol
