GR Safe Nucleic Acid Stain, 1 ml

GR Safe Nucleic Acid Stain: An Excellent Alternative to Ethidium Bromide: Safety, Sensitivity, Stability

Size: 1000 µl, enough for 500 mini gels.

Description: GR Safe is a new, safe nucleic acid stain for detecting double-stranded DNA, single-stranded DNA, and RNA in agarose gel. It can be used for replacing mutagenic ethidium bromide (EB). GR Safe emits green fluorescence when bound to dsDNA and red fluorescence when bound to ssDNA or RNA. This new stain has two fluorescence excitation maxima when bound to nucleic acid, one centered at approximately 290 nm and one at approximately 490nm. GR Safe is as sensitive as EB, and much cheaper than SYBR Green, SYBR Gold and SYBR Safe, and you can use GR Safe just as the way you used EB.

Compared to EB which is a very strong mutagen, GR Safe caused very few mutations in the Ames test. In addition, GR Safe had a negative result in mouse marrow chromophilous erythrocyte micronucleus test and mouse spermary spermatocyte chromosomal aberration test.

• Available at 10,000X in H₂O for better safety --No more toxic and flammable organic solvent
• Room temperature storage for better convenience, stable at room temperature for years --No more freeze-and-thaw cycle!
• As sensitive as Ethidium Bromide and SYBR Safe --With confidence in your mind: it works!.
• Add GR Safe to warm agarose gel solution as you did with ethidium bromide before--No need to do lengthy post electrophoresis staining, save your valuable time.
• DNA bands can be viewed using either UV or safer Blue Light Transilluminator. If you use Blue Light transilluminator, you will not expose to dangerous UV light, so you will not get sun burn or skin cancer or damaged eyes.
• You can use digital camera to take gel photos: No need to use expensive gel documentation equipment or Polaroid Camera and films.
• Will not affect downstream experiments: compatible with all gel purification kits tested, will not inhibit ligation reaction etc.
• Compatible with Sodium Borate Electrophoresis Buffer: Run gel 2-3 times faster at higher voltage, resolve shaper bands in minutes, and less heat generation.
• Cut out DNA bands for subclonning under safer blue light: No mutations caused by EB and UV light.
• Watch DNA migrate at your bench, in real-time without UV light.

Sensitivity:

Example:

 GR Safe vs EtBr

GR Safe	EtBr

Storage: Store at room temperature.

Disposal:

• Gel: Biosafety trash bag.
• TAE or Borate Buffer Solution: sink or consult a chemical safety officer at your institution.

1. Prepare 40 ml of agarose gel solution (concentration from 0.8~2.0%) with TAE or Borate Buffer in a 250 ml flask and mix it thoroughly. Place the flask in the microware, heat on high until the solution is completely clear and no small floating particles are visible (about 2~3 minutes).

3. Pour the gel solution into a gel tray until the comb teeth are immersed about 1/4~1/2 into the gel solution.

5. Detect the bands using UV or blue light transilluminator.

MATERIAL SAFETY DATA SHEET

GR Safe Nucleic Acid Stain 

Physical Description and Properties

Health Effects:

Acute 

 Chronic

First Aid:

The above information is believed to be correct but does not purport to be complete and should be used only as a guide. The burden of safe use of this material rests entirely with the user.