Taq DNA polymerase, Recombinant, 2500 U
Total Units: 2500 units.
CONCENTRATION: 5 units/µl.
UNIT DEFINITION: One unit is defined as the amount of enzyme that incorporates 10 nmoles of dNTPs into acidinsoluble form in 30 minutes at 72 °C under the assay conditions (25 mM TAPS (tris-(hydroxy-methyl)-methyl-amino-pro-panesulfonic acid, sodium salt) pH 9.3 (at 25 °C); 50 mM KCl; 2 mM MgCl2; 1 mM ß-mercaptoethanol) and activated calf thymus DNA as substrate.
STORAGE BUFFER: 10 mM K-phosphate buffer pH 7.0, 100 mM NaCl, 0.5 mM EDTA, 1 mM DTT, 0.01% Tween 20, 50% glycerol(v/v)
STORAGE TEMPERATURE: Store Taq DNA polymerase below 0 °C, preferably at -20 °C, in a constant temperature freezer.
10X REACTION BUFFER: 160 mM (NH 4 ) 2 SO 4 , 670 mM Tris-HCl pH 8.8 (at 25 °C), 15 mM MgCl 2 , 0.1% Tween 20
ASSOCIATED ACTIVITIES: Endonuclease- and exonuclease activities were not detectable after 2 and 1 hours incubation, respectively, of 1 µg lambda DNA and 0.22 µg of EcoRI digested lambda DNA, respectively, at 72 °C in the presence of 15 - 20 units of Taq DNA polymerase.
