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PCR & qPCR

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ÄQStartTM Taq Mastermix

Catalog #: EG-1016

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ÄQStartTM Taq Mastermix

Catalog# EG-1016
Description

2X ÄQStartTM Taq Mastermix is formulated for faster and easier preparation of PCR reactions,  requiring only the addition of primers, templates and water. A reduction in the number of pipetting steps also ensures more reproducible PCR.

ÄQStartTM Taq DNA polymerase contains purified monoclonal antibodies that bind to the polymerase and keep it inactive prior to the initial PCR denaturation step. Upon heat activation (1 minute at 94°C), the antibodies denature irreversibly, releasing fully active, unmodified Taq DNA polymerase. This enables specific and efficient primer extension with the convenience of room temperature reaction assembly.

Applications Routine PCR amplification of up to 4 kb DNA fragment.
 Features
  • High yield, high sensitivity ÄQStart Taq DNA Polymerase
  • Convenient pre-mixed, pre-optimized 2x solutions
  • Hot-start technology ensures specific and efficient primer extension
  • Superior reproducibility and precision
  • Convenient reaction assembly at room temperature
  • Dramatically decreases the time required for reaction set-up
  • Avoids tiresome manipulations such as thawing of enzyme, dilution of enzyme, pipetting of dNTPs and MgCl2.
  • Stable at 4°C
Components Final concentration (1X):
  • Recombinant ÄQStartTM Taq DNA Polymerase: 0.02U/µl
  • dNTPs, each: 100µM
  • Tris-HCl pH9.0: 10mM
  • KCl: 50mM
  • MgCl2: 1.5mM
  • Triton X-100: 0.1%
  • BSA: 0.2mg/ml
  • Glycerol: 1.8%
  • Proprietary PCR enhancer
Storage Conditions -20°C
Stability 3 years at -80 °C;  2 years at -20°C, up to 1 year at 4°C. Repeated freeze/thaw cycles should be avoided but does not affect product performance significantly.
Shipping 4°C
QC

Top Row: Platinum Mix
Bottom Row: ÄQStartTM Taq Mastermix
Template: 1:2 serial dilution pGEM 3z f(+) starting concentration 12.5ng/µl
Note For research use only

 


 
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