UltraHiFiTM Pfu DNA Polymerase [EG-1101] - $0.00 : Lab Supply Mall, Reagents, Life Science Technologies

Please Choose:

Size

Qty:

Description

UltraHiFi^TM Pfu DNA Polymerase is a thermostable DNA polymerase purified from an E. coli strain that expresses the pol gene from Pyrococcus furiosus. It is one of the most thermostable DNA polymerases catalyzing DNA systhesis at optimal temperature near 75°C with exceptional low error rate. UltraHiFi^TM Pfu DNA Polymerase possesses 5´→ 3´ polymerase activity, 3´→ 5´ exonuclease activity and will generate blunt-ended PCR products

Applications

High fidelity PCR amplification
Blunt-end PCR cloning

Features

Low error rate: 1 x 10^-6 to 1 x 10^-7
High speed PCR, extension time can be set to less than 1 kb per minute.
High yield PCR product with 1 unit per reaction.

Components

UltraHiFi^TM Pfu DNA Polymerase, 250 units in strorage buffer: 20mM Tris-HC1, pH 8.2, 1mM DTT, 0.1mM EDTA, 100mM KC1, 0.1% Nonidet P40, 0.1% Tween 20 and 50% glycerol).
10X PCR Buffer with MgSO₄:
200mM Tris-HCl (pH 8.8 at 25°C), 100mM (NH₄)₂SO₄, 100mM KC1, 1% Triton X-100, 1mg/ml BSA, 20mM MgSO₄.

Storage

UltraHiFi^TM Pfu DNA polymerase is stable for 2 years when stored in a constant temperature freezer at -20°C.

Shipping

4°C

Note

For research use only. This product is not available in some countries including USA due to patent protection

Customers who bought this product also purchased...

ÄQStart^TM Taq DNA polymerase

GRGreen Nucleic Acid Stain, 10,000X in DMSO

Ultrapure dNTP Set (dATP, dTTP, dCTP, dGTP), 4x25 umol

Tell a Friend

Write a Review

Price Quote

Home \| Privacy Policy \| Terms & Conditions \| Online Price Policy \| Shipping & Return \| FAQ \| 1 2