Cdks are the catalytic subunits of serine/threonine protein kinases which are involved in the progression through the cell cycle. The catalytic activity of these kinases requires interaction with cyclins, which are synthesized and degraded during the cell cycle in a cyclical fashion. CDK3 associated with Cyclin A, Cyclin C and Cyclin E, participates in the G1-S progression by binding to E2F-1, E2F-2, or E2F-3 through DP-1 and by enhancing their transcriptional activities.
Human cyclin-dependent protein kinase CDK3, coexpressed in complex with Cyclin E. Active protein expressed in Sf9 insect cells and purified as fusion protein by affinity chromatography. Molecular weight based on amino acid sequence 63.9/72.0 kDa (fusion proteins). Specific activity 112.000 pmol/mg x min (filter binding assay).
Size: 20 µg
Ordering information: shipped on dry ice
Product specific literature references:
Yamochi T, Semba K, Tsuji K, Mizumoto K, Sato H, Matsuura Y, Nishimoto I, Matsuoka M. (2001) „ik3-1/Cables is a substrate for cyclin-dependent kinase 3 (cdk 3)." Eur J Biochem. 268(23):6076-82.
Sanchez I, Dynlacht BD. (2005) „New insights into cyclins, CDKs, and cell cycle control."
Semin Cell Dev Biol.,16(3):311-21.
Hofmann F, Livingston DM. (1996) „Differential effects of cdk2 and cdk3 on the control of pRb and E2F function during G1 exit" Genes Dev. 1;10(7):851-61.
Matsuoka M, Matsuura Y, Semba K, Nishimoto I. (2000) „ Molecular cloning of a cyclin-like protein associated with cyclin-dependent kinase 3 (cdk 3) in vivo." Biochem Biophys Res Commun. 5;273(2):442-7. Product Information
