Cdks are the catalytic subunits of serine/threonine protein kinases which are involved in the progression through the cell cycle. The catalytic activity of these kinases requires interaction with cyclins, which are synthesized and degraded during the cell cycle in a cyclical fashion. Induced by mitogenic signals cyclins of the D-type family (D1, D2, and D3) are synthesized, bind and activate CDK4 and CDK6. Active complexes are involved in entering the G1-phase and regulate the early phase of G1 by phosphorylation of the retinoblastoma protein (Rb), p107 and p130. While CDK6 has long been understood only as a mitogenic factor, some studies have indicated that CDK6 can inhibit cell proliferation, suggesting a transcriptional mechanism of CDK6 function in the process of differentiation.
Human cyclin-dependent protein kinase CDK6, coexpressed in complex with Cyclin D1. Active protein expressed in Sf9 insect cells and purified as fusion protein by affinity chromatography. Molecular weight based on amino acid sequence 63.3/60.0 kDa (fusion proteins). Specific activity 14.000 pmol/mg x min (filter binding assay).
Size: 10 µg
Ordering information: shipped on dry ice
Product specific literature references:
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