In response to DNA damage and replication blocks, cells activate pathways that arrest the cell cycle and induce the transcription of genes that facilitate repair. In mammals, ATM (ataxia telangiectasia mutated) kinase together with other checkpoint kinases are important components in this response. Chk2 is the mammalian homolog of the Saccharomyces cerevisiae Rad53 and Schizosaccharomyces pombe Cds1 protein kinases required for the DNA damage and replication checkpoints. Defects in Chk2 contribute to the development of both hereditary and sporadic human cancers, and earmark this kinase as a candidate tumour suppressor and an attractive target for drug discovery.
Affinity-purified recombinant human checkpoint homologue kinase (CHK2) expressed in insect cells featuring an affinity tag. Highly active form of CHK2 suitable for labeling CHK substrates . Specific activity > 25.000 pmoles/min/mg. Molecular weight based on amino acid sequence 66 kDa.
Size: 5 µg
Ordering information: shipped on dry ice
Product specific literature references:
Matsuoka S, Huang M, Elledge SJ (1998) "Linkage of ATM to cell cycle regulation by the Chk2 protein kinase" Science 282(5395):1893-7
Chaturvedi P, Eng WK, Zhou BB et al. (1999) "Mammalian Chk2 is a downstream effector of the ATM-dependent DNA damage checkpoint pathway" Oncogene 18(28):4047-54
Matsuoka S, Rotman G, Ogawa A, Shiloh Y, Tamai K, Elledge SJ (2000) "Ataxia telangiectasia-mutated phosphorylates Chk2 in vivo and in vitro" Proc. Natl. Acad. Sci. U S A 97(19):10389-94
Melchionna R, Chen XB, Blasina A, McGowan CH (2000) "Threonine 68 is required for radiation-induced phosphorylation and activation of Cds1" Nat. Cell. Biol. 2(10):762-5
Chehab NH, Malikzay A, Appel M, Halazonetis TD (2000) "Chk2/hCds1 functions as a DNA damage checkpoint in G(1) by stabilizing p53" Genes Dev. 14(3):278-88
Falck J, Mailand N, Syljuasen RG, Bartek J, Lukas J (2001) "The ATM-Chk2-Cdc25A checkpoint pathway guards against radioresistant DNA synthesis" Nature 410(6830):842-7
Bartek J, Falck J, Lukas J (2001) "CHK2 kinase--a busy messenger" Nat. Rev. Mol. Cell. Biol. 2(12):877-86
McGowan CH (2002) "Checking in on Cds1 (Chk2): A checkpoint kinase and tumor suppressor" Bioessays 24(6):502-11
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