Human casein kinase 2 beta (hCK2 beta). The beta subunbit is the non-catalytic subunit of the serine/threonine protein kinase CK2 possibly interacting with other protein kinases. The beta subunit itself does not have kinase activity, but confers stability to the CK2 alpha subunit and is involved in activity and substrate specificity. CK2 beta is recombinantly expressed in E. coli and purified as a non-fusion protein using several chromatographic steps. Mw 24,9 kDa. Purity >98% by SDS-PAGE, no protease activity detectable.
Size: 10 µg
Ordering information: shipped on dry ice
Product specific literature references:
Heller-Harrison RA, Meisner H, Czech MP (1989) "Cloning and characterization of a cDNA encoding the beta subunit of human casein kinase II" Biochemistry 28(23):9053-8
Voss H, Wirkner U, Jakobi R, Hewitt NA, Schwager C, Zimmermann J, Ansorge W, Pyerin W (1991) "Structure of the gene encoding human casein kinase II subunit beta" J. Biol. Chem. 266(21):13706-11
Meggio F, Boldyreff B, Marin O, Pinna LA, Issinger OG (1992) "Role of the beta subunit of casein kinase-2 on the stability and specificity of the recombinant reconstituted holoenzyme" Eur. J. Biochem. 204(1):293-7
Boldyreff B, Meggio F, Pinna LA, Issinger OG (1992) "Casein kinase-2 structure-function relationship: creation of a set of mutants of the beta subunit that variably surrogate the wildtype beta subunit function" Biochem. Biophys. Res. Commun. 188(1):228-34
Robitzki A, Bodenbach L, Voss H, Pyerin W (1993) "Human casein kinase II. The subunit alpha protein activates transcription of the subunit beta gene" J. Biol. Chem. 268(8):5694-702
Boldyreff B, Meggio F, Pinna LA, Issinger OG (1994) "Efficient autophosphorylation and phosphorylation of the beta-subunit by casein kinase-2 require the integrity of an acidic cluster 50 residues downstream from the phosphoacceptor site" J. Biol. Chem. 269(7):4827-31
Marin O, Meggio F, Boldyreff B, Issinger OG, Pinna LA (1995) "Dissection of the dual function of the beta-subunit of protein kinase CK2 ('casein kinase-2'): a synthetic peptide reproducing the carboxyl-terminal domain mimicks the positive but not the negative effects of the whole protein" FEBS Lett. 363(1-2):111-4
Gietz RD, Graham KC, Litchfield DW (1995) "Interactions between the subunits of casein kinase II" J. Biol. Chem. 270(22):13017-21
Dobrowolska G, Lozeman FJ, Li D, Krebs EG (1999) "CK2, a protein kinase of the next millennium" Mol. Cell. Biochem. 191(1-2):3-12
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