Zea mays casein kinase 2 alpha (Z.m. CK2 alpha), recombinantly expressed in E. coli, purified using several chromatographic steps. Mw 39,2 kDa, purity >99% by SDS-PAGE, no protease activity detectable, specific activity 1.000.000 U/mg (1 U = 1 pmol/min at 30 degree C) using the substrate peptide RRRDDDSDDD.
Size: 10 µg
Ordering information: shipped on dry ice
Product specific literature references:
Niefind K, Guerra B, Pinna LA, Issinger OG, Schomburg D (1998) "Crystal structure of the catalytic subunit of protein kinase CK2 from Zea mays at 2.1 A resolution" EMBO J. 17(9):2451-62
Battistutta R, Sarno S, De Moliner E, Marin O, Issinger OG, Zanotti G, Pinna LA (2000) "The crystal structure of the complex of Zea mays alpha subunit with a fragment of human beta subunit provides the clue to the architecture of protein kinase CK2 holoenzyme" Eur. J. Biochem. 267(16):5184-90
Riera M, Peracchia G, Pages M (2001) "Distinctive features of plant protein kinase CK2" Mol. Cell. Biochem. 227(1-2):119-27
Riera M, Pages M, Issinger OG, Guerra B (2003) "Purification and characterization of recombinant protein kinase CK2 from Zea mays expressed in Escherichia coli" Protein Expr. Purif. 29(1):24-32
Riera M, Figueras M, Lopez C, Goday A, Pages M (2004) "Protein kinase CK2 modulates developmental functions of the abscisic acid responsive protein Rab17 from maize" Proc. Natl. Acad. Sci. U S A. 101(26):9879-84
Product Information
