Human ERK1, recombinantly expressed in E. coli. Unactive form not phosphorylated by MEK1. Molecular weight based on amino acid sequence 43.6 kDa. Purity > 95% (SDS PAGE). Specific activity < 200 Units/mg. No protease activity detectable.
Alternate Names: p44, p44 MAPK, p44MAPK, mitogen-activated protein kinase 3, MAP kinase 3, MAPK3, extracellular signal-regulated kinase 1, p44 ERK1, p44ERK1
Size: 20 µg
Ordering information: shipped on dry ice
Product specific literature references:
Seger R, Ahn NG, Krebs EG et al. (1991) "Microtubule-associated protein 2 kinases, ERK1 and ERK2, undergo autophosphorylation on both tyrosine and threonine residues: implications for their mechanism of activation" Proc Natl Acad Sci USA 88(14):6142-6
Gonzalez FA, Raden DL, Davis RJ (1991) "Identification of substrate recognition determinants for human ERK1 and ERK2 protein kinases" J. Biol. Chem. 266(33):22159-63
Haycock JW, Ahn NG, Cobb MH, Krebs EG (1992) "ERK1 and ERK2, two microtubule-associated protein 2 kinases, mediate the phosphorylation of tyrosine hydroxylase at serine-31 in situ" Proc. Natl. Acad. Sci. U S A. 89(6):2365-9
Meloche S, Pages G, Pouyssegur J (1992) "Functional expression and growth factor activation of an epitope-tagged p44 mitogen-activated protein kinase, p44mapk" Mol Biol Cell 3(1):63-71
Cook SJ, Rubinfeld B, Albert I, McCormick F (1993) "RapV12 antagonizes Ras-dependent activation of ERK1 and ERK2 by LPA and EGF in Rat-1 fibroblasts" EMBO J. 12(9):3475-85
Pages G, Lenormand P, Pouyssegur J et al (1993) "Mitogen-activated protein kinases p42mapk and p44mapk are required for fibroblast proliferation" Proc Natl Acad Sci U S A 90(18):8319-23
Lenormand P, Sardet C, Pouyssegur J et al (1993) "Growth factors induce nuclear translocation of MAP kinases (p42mapk and p44mapk) but not of their activator MAP kinase kinase (p45mapkk) in fibroblasts" J Cell Biol. 122(5):1079-88
Volente C, Angelastro JM, Greene LA (1993) "Association of protein kinases ERK1 and ERK2 with p75 nerve growth factor receptors" J. Biol. Chem. 268(28):21410-5
Hartsough MT, Mulder KM (1995) "Transforming growth factor beta activation of p44mapk in proliferating cultures of epithelial cells" J Biol Chem. 270(13):7117-24
Songyang Z, Lu KP, Cantley LC et al. (1996) "A structural basis for substrate specificities of protein Ser/Thr kinases: primary sequence preference of casein kinases I and II, NIMA, phosphorylase kinase, calmodulin-dependent kinase II, CDK5, and Erk1" Mol Cell Biol. 16(11):6486-93
Veeranna, Amin ND, Ahn NG, Jaffe H, Winters CA, Grant P, Pant HC (1998) "Mitogen-activated protein kinases (Erk1,2) phosphorylate Lys-Ser-Pro (KSP) repeats in neurofilament proteins NF-H and NF-M" J. Neurosci. 18(11):4008-21
Pages G, Guerin S, Pouyssegur J (1999) "Defective thymocyte maturation in p44 MAP kinase (Erk 1) knockout mice" Science 286(5443):1374-7
Brondello JM, Pouyssegur J, McKenzie FR (1999) "Reduced MAP kinase phosphatase-1 degradation after p42/p44MAPK-dependent phosphorylation" Science 286(5449):2514-7
Lu Z, Xu S, Joazeiro C, Cobb MH, Hunter T (2002) "The PHD domain of MEKK1 acts as an E3 ubiquitin ligase and mediates ubiquitination and degradation of ERK1/2" Mol Cell 9(5):945-56
Mazzucchelli C, Vantaggiato C, Brambilla R et al. (2002) "Knockout of ERK1 MAP kinase enhances synaptic plasticity in the striatum and facilitates striatal-mediated learning and memory" Neuron 34(5):807-20
Product Information
