Abl1 belongs to the family of the non-receptor tyrosine kinases. The protein is ubiquitously expressed in cells of various tissues at the cytoplasmic as well as nuclear level and is involved in the regulation of cell growth, survival and morphogenesis. Abl proteins show a modular organization, similar to that of members of the Src family, and are characterized by an N-terminus followed by a Src homology 3 (SH3) domain, a SH2 domain and the catalytic core. By intramolecular interactions Abl1 is kept in a closed and inactive conformation. Activation occurs upon interaction with SH3- and/or SH2-binders, which induces an opening of the structure. Deregulated Abl1 is involved in cancer. The translocation of ABL1 to the BCR gene, generating the Bcr–Abl fusion, the so called Philadelphia (Ph)-chromosome, results in a constitutive active tyrosine-kinase which is strongly associated with chronic myeloid leukemia (CML).
Size: 20 µg
Specific activity : 33.000 pmol/mg x min
Method for determination of Km value & specific activity: Filter binding assay MAFC membrane
Protein concentration: 0.206 mg/ml (Bradford method using BSA as standard protein)
Ordering information: shipped on dry ice
Product specific literature:
Saglio G, Cilloni D. (2004) " Abl: the prototype of oncogenic fusion proteins." Cell Mol Life Sci. 61(23):2897-911.
Sirvent A, Benistant C, Roche S. (2008) "Cytoplasmic signalling by the c-Abl tyrosine kinase in normal and cancer cells." Biol Cell. 100(11):617-31.
Wang JY. (1993) "Abl tyrosine kinase in signal transduction and cell-cycle regulation." Curr Opin Genet Dev. 3(1):35-43.
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