Human, JNK2 alpha / SAPK1 alpha, JNK2 / SAPK1.
Human JNK2a/SAPK1a, recombinantly expressed in E. coli. Molecular weight based on amino acid sequence 49.5 kDa. Purity > 95% (SDS PAGE). Specific activity > 300 U*/mg (*1 U = 1 pmol/min transferred to c-jun (1-169) at 30 degree C). Autophosphorylation < 5%. No protease activity detectable.
Size: 100 µg
Ordering information: shipped on dry ice
Product specific literature references:
Sluss HK, Barrett T, Derijard B, Davis RJ (1994) "Signal transduction by tumor necrosis factor mediated by JNK protein kinases" Mol Cell Biol. 14(12):8376-84
Kallunki T, Su B, Tsigelny I, Sluss HK, Derijard B, Moore G, Davis R, Karin M (1994) "JNK2 contains a specificity-determining region responsible for efficient c-Jun binding and phosphorylation" Genes Dev. 8(24):2996-3007
Uciechowski P, Saklatvala J, von der Ohe J, Resch K, Szamel M, Kracht M (1996) "Interleukin 1 activates jun N-terminal kinases JNK1 and JNK2 but not extracellular regulated MAP kinase (ERK) in human glomerular mesangial cells" FEBS Lett. 394(3):273-8
Yang DD, Conze D, Whitmarsh AJ, Barrett T, Davis RJ, Rincon M, Flavell RA (1998) "Differentiation of CD4+ T cells to Th1 cells requires MAP kinase JNK2" Immunity 9(4):575-85
Sabapathy K, Hu Y, Kallunki T, Schreiber M, David JP, Jochum W, Wagner EF, Karin M (1999) "JNK2 is required for efficient T-cell activation and apoptosis but not for normal lymphocyte development" Curr Biol. 9(3):116-25
Kuan CY, Yang DD, Samanta Roy DR, Davis RJ, Rakic P, Flavell RA (1999) "The Jnk1 and Jnk2 protein kinases are required for regional specific apoptosis during early brain development" Neuron. 22(4):667-76
Ham J, Eilers A, Whitfield J, Neame SJ, Shah B (2000) "c-Jun and the transcriptional control of neuronal apoptosis" Biochem. Pharmacol. 60(8):1015-21
Hochedlinger K, Wagner EF, Sabapathy K (2002) "Differential effects of JNK1 and JNK2 on signal specific induction of apoptosis" Oncogene 21(15):2441-5
Arbour N, Naniche D, Homann D, Davis RJ, Flavell RA, Oldstone MB (2002) "c-Jun NH(2)-terminal kinase (JNK)1 and JNK2 signaling pathways have divergent roles in CD8(+) T cell-mediated antiviral immunity" J Exp Med. 195(7):801-10
Bogoyevitch MA, Boehm I, Oakley A, Ketterman AJ, Barr RK (2004) "Targeting the JNK MAPK cascade for inhibition: basic science and therapeutic potential" Biochim Biophys Acta. 1697(1-2):89-101
Sabapathy K, Hochedlinger K, Nam SY, Bauer A, Karin M, Wagner EF (2004) "Distinct roles for JNK1 and JNK2 in regulating JNK activity and c-Jun-dependent cell proliferation" Mol Cell. 15(6):843-4
Tafolla E, Wang S, Wong B, Leong J, Kapila YL (2005) "JNK1 and JNK2 Oppositely Regulate p53 in Signaling Linked to Apoptosis Triggered by an Altered Fibronectin Matrix: JNK LINKS FAK AND p53" J Biol Chem. 280(20):19992-9
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