Human mitogen activated kinase kinase 1, recombinant, active.
Mitogen-activated protein (MAP) kinases require dual phosphorylation on threonine and tyrosine residues in order to gain enzymatic activity. This activation is carried out by a family of enzymes known as MAP kinase kinases (MKKs or MEKs).
Human recombinant mitogen activated protein kinase kinase 1 (MEK1, MKK1, MAPKK1, MAP2K1), expressed in E. coli. Highly active and purified form which is activated by inserting two amino acid exchanges (S218D, S222D). Suitable for phosphorylation of MEK1 substrates and activation of ERK1 and ERK2. Features a fusion tag to facilitate removal of the enzyme from reaction mixtures. Molecular weight based on amino acid sequence 69.7 kDa. Purity > 80% (SDS PAGE). Specific activity > 250.000 U*/mg (*1 U = 1 pmol/min transferred to inactive ERK1 at 30 degree C).
Size: 10 µg
Ordering information: shipped on dry ice
Product specific literature references:
Huang W, Kessler DS, Erikson RL (1995) "Biochemical and biological analysis of Mek1 phosphorylation site mutants" Mol Biol Cell. 6(3):237-45
Alessandrini A, Greulich H, Huang W, Erikson RL (1996) "Mek1 phosphorylation site mutants activate Raf-1 in NIH 3T3 cells" J Biol Chem. 271(49):31612-8
Schramek H, Feifel E, Healy E, Pollack V. (1997) "Constitutively active mutant of the mitogen-activated protein kinase kinase MEK1 induces epithelial dedifferentiation and growth inhibition in madin-darby canine kidney-C7 cells" J Biol Chem. 272(17):11426-33
Cheng M, Sexl V, Sherr CJ, Roussel MF (1998) "Assembly of cyclin D-dependent kinase and titration of p27Kip1 regulated by mitogen-activated protein kinase kinase (MEK1)" Proc Natl Acad Sci U S A. 95(3):1091-6
Alessandrini A, Namura S, Moskowitz MA, Bonventre JV (1999) "MEK1 protein kinase inhibition protects against damage resulting from focal cerebral ischemia" Proc Natl Acad Sci U S A 96(22):12866-9
Giroux S, Tremblay M, Charron J et al. (1999) "Embryonic death of Mek1-deficient mice reveals a role for this kinase in angiogenesis in the labyrinthine region of the placenta" Curr Biol. 9(7):369-72
Allen LF, Sebolt-Leopold J, Meyer MB (2003) "CI-1040 (PD184352), a targeted signal transduction inhibitor of MEK (MAPKK)" Semin Oncol. 30(5 Suppl 16):105-16
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