Human p38 / SAPK2 alpha, recombinant, unactive
Human p38 alpha / SAPK2 alpha, recombinantly expressed in E. coli. Inactive form of p38 alpha / SAPK2 alpha, which is not activated by phosphorylation by MKK6 and thus serves as a negative control for p38 / SAPK2 phosphorylation. Molecular weight based on amino acid sequence 42.7 kDa. Purity > 95% (SDS PAGE).
Size: 20 µg
Ordering information: shipped on dry ice
Product specific literature references:
Schwenger P, Bellosta P, Vilcek J et al (1997) "Sodium salicylate induces apoptosis via p38 mitogen-activated protein kinase but inhibits tumor necrosis factor-induced c-Jun N-terminal kinase/stress-activated protein kinase activation" Proc Natl Acad Sci U S A. 94(7):2869-73
Deak M, Clifton AD, Lucocq LM, Alessi DR (1998) "Mitogen- and stress-activated protein kinase-1 (MSK1) is directly activated by MAPK and SAPK2/p38, and may mediate activation of CREB" EMBO J. 17(15):4426-41
Huot J, Houle F, Rousseau S, Deschesnes RG, Shah GM, Landry J (1998) "SAPK2/p38-dependent F-actin reorganization regulates early membrane blebbing during stress-induced apoptosis" J Cell Biol. 143(5):1361-73
Rolli M, Kotlyarov A, Sakamoto KM, Gaestel M, Neininger A (1999) "Stress-induced stimulation of early growth response gene-1 by p38/stress-activated protein kinase 2 is mediated by a cAMP-responsive promoter element in a MAPKAP kinase 2-independent manner" J Biol Chem. 274(28):19559-64
Rolli-Derkinderen M, Gaestel M (2000) "p38/SAPK2-dependent gene expression in Jurkat T cells" Biol Chem. 381(3):193-8
Ono K, Han J (2000) "The p38 signal transduction pathway: activation and function" Cell Signal. 12(1):1-13
Casanovas O, Miro F, Estanyol JM, Itarte E, Agell N, Bachs O (2000) "Osmotic stress regulates the stability of cyclin D1 in a p38SAPK2-dependent manner" J Biol Chem. 275(45):35091-7
Laferriere J, Houle F, Taher MM, Valerie K, Huot J (2001) "Transendothelial migration of colon carcinoma cells requires expression of E-selectin by endothelial cells and activation of stress-activated protein kinase-2 (SAPK2/p38) in the tumor cells" J Biol Chem. 276(36):33762-72
Kumar S, Boehm J, Lee JC (2003) "p38 MAP kinases: key signalling molecules as therapeutic targets for inflammatory diseases" Nat Rev Drug Discov. 2(9):717-26
Shin J, Park B, Ahn K eta al. (2004) "Promyelocytic leukemia is a direct inhibitor of SAPK2/p38 mitogen-activated protein kinase" J Biol Chem. 279(39):40994-1003
Saklatvala J (2004) "The p38 MAP kinase pathway as a therapeutic target in inflammatory disease" Curr Opin Pharmacol. 4(4):372-7
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