high quality PKA catalytic subunit, type alpha human (PKA Calpha)
Highly active PKA catalytic subunit recombinantly expressed in E. coli and purified using several chromatographic steps. Monomeric enzyme (Mw 41 kDa) preferentially phosphorylating substrates with recognition sites like RxxS, xRxS, RRxT, KRxS, RKxS and similar. Preferred peptide substrate is kemptide (LRRASLG).
enzymatic activity > 10.000.000 units*/mg (based on kemptide phosphorylation), purity >95% (SDS-PAGE)
*1 unit is defined as 1 pmol phosphate per mg and minute.
Size: 50 µg
Ordering information: shipped on dry ice
Product specific literature references:
Taylor SS (1989) "cAMP-dependent protein kinase. Model for an enzyme family" J. Biol. Chem. 264(15):8443-6
Taylor SS, Buechler JA, Yonemoto W (1990) "cAMP-dependent protein kinase: framework for a diverse family of regulatory enzymes" Annu. Rev. Biochem. 59:971-1005
Walsh DA, Glass DB, Mitchell RD (1992) "Substrate diversity of the cAMP-dependent protein kinase: regulation based upon multiple binding interactions" Curr. Opin. Cell Biol. 4(2):241-51
Sculptoreanu A, Rotman E, Takahashi M, Scheuer T, Catterall WA (1993) "Voltage-dependent potentiation of the activity of cardiac L-type calcium channel alpha 1 subunits due to phosphorylation by cAMP-dependent protein kinase" Proc. Natl. Acad. Sci. U S A 90 (21): 10135-9
Walsh DA, Van Patten SM (1994) "Multiple pathway signal transduction by the cAMP-dependent protein kinase" FASEB J. 8(15):1227-36
Tasken K, Skalhegg BS, Jahnsen T et al. (1997) "Structure, function, and regulation of human cAMP-dependent protein kinases" Adv. Second Messenger Phosphoprotein Res. 31:191-204
Gray PC, Scott JD, Catterall WA (1998) "Regulation of ion channels by cAMP-dependent protein kinase and A-kinase anchoring proteins" Curr. Opin. Neurobiol. 8(3):330-4
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