Protein kinase B or Akt (PKB/Akt) is a serine/threonine kinase, which in mammals comprises three highly homologous members known as PKB alpha (Akt1), PKB beta (Akt2) and PKB gamma (Akt3). PKB/Akt is a growth-factor-regulated protein kinase which contains a pleckstrin homology (PH) domain. Binding of phosphoinositide 3-OH kinase products to the pleckstrin homology domain results in translocation of PKB/Akt to the plasma membrane where it is activated by phosphorylation by upstream kinases including the phosphoinoside-dependent kinase 1 (PDK1). Key roles for this enzyme can be found in cellular processes such as glucose metabolism, cell proliferation, apoptosis, transcription and cell migration.
Human protein kinase Akt1/PKBalpha, recombinantly expressed. Unactive enzyme, suitable for negative control experiments or for phosphorylation as a substrate. The inactive Akt1/PKBalpha is not phosphorylated at Thr308 and Ser473 and can be used to test phosphorylation of these two sites. Mw 59,1 kDa. Purity >95% by SDS-PAGE.
Size: 20 µg
Ordering information: shipped on dry ice
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