Akt2/PKB beta, active
[PKBB-A040]
Protein kinase B or Akt (PKB/Akt) is a serine/threonine kinase, which in mammals comprises three highly homologous members known as PKB alpha (Akt1), PKB beta (Akt2) and PKB gamma (Akt3). PKB/Akt is a growth-factor-regulated protein kinase which contains a pleckstrin homology (PH) domain. Binding of phosphoinositide 3-OH kinase products to the pleckstrin homology domain results in translocation of PKB/Akt to the plasma membrane where it is activated by phosphorylation by upstream kinases including the phosphoinoside-dependent kinase 1 (PDK1). Key roles for this enzyme can be found in cellular processes such as glucose metabolism, cell proliferation, apoptosis, transcription and cell migration
Human protein kinase Akt2/PKB, recombinantly expressed. Mw 60,4 kDa. Purity >95% by SDS-PAGE. Specific activity > 4.000 Units/mg (1 Unit = 1 pmol/min transferred to synthetic peptide RPRAATF at 30 degree Celsius). No protease activity detectable.
Size: 40 µg
Ordering information: shipped on dry ice
Product specific literature references:
Walker KS, Deak M, Paterson A, Hudson K, Cohen P, Alessi DR (1998) "Activation of protein kinase B beta and gamma isoforms by insulin in vivo and by 3-phosphoinositide-dependent protein kinase-1 in vitro: comparison with protein kinase B alpha" Biochem. J. 331 ( Pt 1):299-308
Okano J, Gaslightwala I, Birnbaum MJ, Rustgi AK, Nakagawa H (2000) "Akt/protein kinase B isoforms are differentially regulated by epidermal growth factor stimulation" J. Biol. Chem. 275(40):30934-42
Cho H, Mu J, Kim JK, Thorvaldsen JL, Chu Q, Crenshaw EB 3rd, Kaestner KH, Bartolomei MS, Shulman GI, Birnbaum MJ (2001) "Insulin resistance and a diabetes mellitus-like syndrome in mice lacking the protein kinase Akt2 (PKB beta)" Science 292(5522):1728-31
Vandromme M, Rochat A, Meier R, Carnac G, Besser D, Hemmings BA, Fernandez A, Lamb NJ (2001) "Protein kinase B beta/Akt2 plays a specific role in muscle differentiation" J. Biol. Chem. 276(11):8173-9
Garofalo RS, Orena SJ, Rafidi K, Torchia AJ, Stock JL, Hildebrandt AL, Coskran T, Black SC, Brees DJ, Wicks JR, McNeish JD, Coleman KG (2003) "Severe diabetes, age-dependent loss of adipose tissue, and mild growth deficiency in mice lacking Akt2/PKB beta" J. Clin. Invest. 112(2):197-208
Bae SS, Cho H, Mu J, Birnbaum MJ (2003) "Isoform-specific regulation of insulin-dependent glucose uptake by Akt/protein kinase B" J. Biol. Chem. 278(49):49530-6
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