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GeneExpresso™ DNA in vitro Transfection Reagent

Catalog #: IV-1031

$228.00  $168.00
- $60.00

Qty:
Size  1 ml
Description GeneExpresso™ is formulated with a novel cationic polymer covalently conjugated to cationic lipid. This reagent has exceptional transfection efficiency with extremely low cytotoxicity. GeneExpresso™ has been used by scientists  to transfect various established cell lines as well as primary cells including HEK293, 293T, 293E, CHO, COS1, HeLa, NIH 3T3, insect cell lines (Sf9 and Sf21) and a variety of other eucaryotic cell lines. 

  • Efficient for difficult-to-transfect cells
  • Transfect a broad range of mammalian cells
  • Low cytotoxicity
  • No sera interference
  • Very stable
  • Simple protocol
  • Suitable for high-thorough application
Broad Transfection Spectrum for Mammalian Cell Types

Cell Lines

Efficiency (% GFP)

Cell Lines

Efficiency (% GFP)

3LL
B16-F10
BAEC
BHK-21
Ca Ski
CaCo2
CHO
CLBPEC
COS-7
CV-1
D 407
DHD Pro.b
HCS-2/8
HEK293
HeLa
HLMEC
H-MVEC
Huh-7D12
ATT20

50 %
73%
51%
82%
70%
31%
81%
10%
70%
51%
61%
53%
41%
85%
80%
60%
50%
23%
39%

K562
L929
MCF-7
MDCK
Neuro2A
NIH 3T3
PC12
Primary human keratinocytes
Primary human pre-adipocytes
Primary human skin fibroblasts
SAOS-2
SHEP
SH-SY5Y
SiHa
SKOV3
HUVEC
IGROV1
Jurkat
6CSFMEo

25%
54%
53%
54%
71%
80%
31%
25%
25%
31%
51%
70%
15%
63%
53%
52%
22%
5%-15%
79%

  

Lipofectamine 2000 GeneExpresso
FACS Analysis

Fig. 1. Efficiency comparison of GeneExpresso and Lipofectamine 2000 on Hela cells.

 

 

GeneExpresso 8000 GeneExpresso
PolyExpress

Fig 2, Transfection of  Glioma Xenograft Cells using GeneExpresso and PolyExpress (Courtesy of Dr. Chandram Chetty, University of Illinois). 

Storage Conditions Upon arrival store this product at 4 oC. If stored properly, the product is stable for 18 months or longer. Product shipped at ambient temperature.
Protocol DNA Transfection Protocol
Notes

For research use only

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