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GeneExpresso™ Plus DNA In Vitro Tranfection Reagent

GeneExpresso™ Plus DNA In Vitro Tranfection Reagent

Catalog #: IV-1032

$238.00 $208.00
- $30.00

Qty:

Size	1 ml
Description	GeneExpresso™ Plus DNA In Vitro Tranfection Reagent is the enhanced version of GeneExpresso™ DNA In Vitro Transfection Reagent. GeneExpresso™ Plus is formulated by addition of a booster peptide, giving a rise up to 20x higher efficiency on some mammalian cells in comparison to it's previous version. GeneExpresso™ Plus was shown to efficiently deliver genes to various established cell lines as well as primary cells including HEK293, 293T, 293E, CHO, COS1, HeLa, NIH 3T3, insect cell lines (Sf9 and Sf21) and a variety of other eucaryotic cell lines with less toxicity. GeneExpresso™ Plus reagent, 1.0 ml, is sufficient for 300 to 600 transfections in 24 well plates or 150 to 300 transfections in 6 well plates.
Advanteges	Cell-dependent 3~20 times higher efficiency Top choice for hard-to-transfect cells Equally good for very long DNAs (up to 2.0 mb) Efficiency boosted in the presence of serum and antibiotics for most of cell types Exceptional high levels of recombinant protein production Comparisons of Transfection Efficiency of GeneExpresso™ Plus In Vitro DNA Transfection Reagent with the Leading Brand Name Products GeneExpresso™ Plus is the top choice for hard-to-transfect cells. Efficiency comparison of GeneExpresso™ Plus In Vitro DNA Transfection Reagent (left panel) with Lipofectamine 2000 (L2K, right panel) on primary cultured rat aortic smooth muscle cells. A nuclear targeted GFP DNA was transfected with GeneExpresso™ Plus and L2K respectively per manufacturer's protocols. The efficiency was checked 48 hours post transfection. Left Panel: Comparison of GeneExpresso™ Plus In Vitro DNA Transfection Reagent with leading products including Lipofectamine 2000 (L2K) and Lipofectamine LTX (LTX) on lentivirus production on 293T cells. Right panel: Comparison of GeneExpresso™ Plus In Vitro DNA Transfection Reagent with tranfection reagents from other vendors inluding Lipofectamine 2000 (L2K) and FuGene 6 on Hela cells. HeLa cells were transfected with plasmid DNA encoding GFP. Transfections were performed according to manufacturers recommendations and analysed by flow cytometry. Exceptionally high titers of lentivirus generated from 293T cells. Three DNAs co-transfected with GeneExpresso™ Plus In Vitro DNA Transfection Reagent (left panel) in comparison with Lipofectamine LTX (right panel). 1.0 and 10 microliters virus supernatant from 293T cells were used to counter-infected 293T cells which was then passed FACS. The numbers at the upper right corner indicate the percentage of transduced cells. The titers of lentivirus generated with GeneExpresso™ Plus and Lipofectamine LTX were quantified to be 2.1x10⁶and 9.3x10⁶ tu/ml respectively.
Protocol	DNA Transfection Protocol
Storage Conditions	Upon arrival store this product at 4 ^oC. If stored properly, the product is stable for 6 months or longer. Product shipped at ambient temperature or with blue ice.
Notes	For research use only
Free Sample	Request a Free Sample

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