Description
PolyExpress™ DNA In Vitro Tranfection Reagent is a biodegradable polymer based DNA transfection reagent. It is effective for transfecting of common (HEK293, COS-7, NIH-3T3, HeLa, CHO) and a broad ranges of hard-to-transfect mammalian cells. PolyExpress™ reagent is capable to immobilize DNA migration during electrophoresis at very low concentration and form polyplexes within a few minutes at room temperature. Due to its biodegrable feature, the cationic polymer is rapidly degraded shortly after entering cells by endocytosis, therefore, it has much less cytotoxicity than other lipisome-based transfection reagent.
Features:
- Bio-degradable after endocytosis
- Exceptional high titers of virus production
- Efficient for very long DNAs (>89 kb)
- Efficient for both single DNA transfection and multi DNA co-transfection
- High levels of recombinant protein production
- Simple and robust transfection procedure
- Very affordable
Storage Condition: Store at 4 °C. If stored properly, the product is stable for 12 months or longer.
Broad Transfection Spectrum for Mammalian Cell Types
|
Cell Lines
|
Efficiency (% GFP)
|
Cell Lines
|
Efficiency (% GFP)
|
| McArdle 7777 |
65 -70% |
SAOS-2 |
58% |
| Hep3D |
67-76% |
SN56 |
81% |
| SHEP |
68-71% |
MC3T3-E1 |
80% |
| 3T3-442A |
35% |
Primary melanocyte |
35% |
| COS-7 |
85-90% |
K562 |
38% |
| CV-1 |
60% |
L929 |
59% |
| D 407 |
70% |
MCF-7 |
68% |
| DHD Pro.b |
70% |
MDCK |
68% |
| 3LL |
80% |
Neuro2A |
86% |
| B16-F10 |
85% |
NIH 3T3 |
76% |
| BAEC |
51% |
PC12 |
30% |
| BHK-21 |
80% |
SH-SY5Y |
25% |
| Ca Ski |
88% |
SiHa |
60% |
| CaCo2 |
60% |
SKOV3 |
65% |
| CHO |
88% |
Huh-7 |
70% |
| HCS-2/8 |
61% |
IGROV1 |
35% |
| HEK-293 |
86% |
DF-1, C hicken Embryonic Cell |
50% |
| HeLa |
88% |
6CSFMEo |
71% |
| HLMEC |
72% |
WEHI 231 |
26% |
| H-MVEC |
59% |
A549 |
75% |
| Huh-7D12 |
72% |
LNCap |
75% |
| ATT20 |
46% |
Prim. mouse keratinocyte |
29% |
| SK-N-SH |
29% |
Prim. human skin fibroblast |
50% |
| C2C12 |
46% |
Prim. human pre-adipocyte |
32% |
| HepG2 |
72% |
Prim. mouse embry. fibroblast |
30% |
Lipofectamine 2000 PolyExpress
Fig 1, Comparison of PolyExpress and Lipofectamine 2000 on 293 Cell.
| GeneExpresso 8000 |
GeneExpresso |
PolyExpress |
Fig 2, Transfection of Glioma Xenograft Cells using GeneExpresso and PolyExpress
(Courtesy of Dr. Chandram Chetty, University of Illinois).
Transfection Protocol
